ISO 8036-1:1998 download.Optics and optical instruments — Microscopes — Part 1: Immersion oI for general use in light microscopy.
Introduction
The image quality of microscopes wttn oII-Immer5lon objectives is dependent on the optical properties of the immersion oil used. In order to guarantee a certain minimum quality, this part of ISO 8036 specifies the relevant properties of immersion oil for general use, with the purpose of making immersion oil with the specified properties available throughout the world.
The optical and physical requirements for immersion oil have been specified taking into account the optical properties of objectives which are in general use and which are made by different manufacturers.
This part of ISO 8036 specifies requirements for immersion oil for general use in light microscopy in the visible spectral range.
NOTE 1 Visible spectral range is defined as being the wavelength range from 400 nm to 760 nm.
Owing to its optical effect, immersion oil forms part of the optical system of a microscope; this part of ISO 8036 specifies the requirements which shall be met to ensure image quality.
NOTE 2 This part of ISO 8036 does not cover requirements for suitability for fluorescence microscopy: immersion oil suitable for fluorescence microscopy Will be dealt with in ISO 8036-2.
2 Requirements
2.1 OptIcal properties
Immersion oil shall have the following optical properties, measured at a temperature of 23 C and at a pressure of 101 325 Pa:
— Principal refractive index: n, = 1.5180 ± 0.000 5
— Abbe number: v1— 44 ± 3
The Abbe number v is calculated using the following formula:
where
vi, is the principal refractive index;
is the refractive index of light at the blue cadmium F’•line (A =479,99 nm);
n is the refractive index of light at the red cadmium C’-line (A., — 643,85 nm).
NOTE 2 The principal refractive index, n, is the refractive index of light at the green mercury e-Iine (A = 546,07 nm; see ISO 7944). This wavelength is close to the maximum sensitivity of the eye and has been used as the principal wavelength of optical computation for some time.
22 Light transmittance
Immersion oil shall have no re9ions of selective absorption between 400 nm and 760 nm. The minimum percentage transmittance measured through a 10 mm cell path shall be as given in table 1.
2.3 Viscosity
Immersion oil shall range in viscosity from 0,000 15 m1/s to 0.001 5 m2ls ± 10 %. measured at a temperature of
23 °C.
2.4 ToxicIty
The data sheet on material safety shall be available from the manufacturer.
3 Marking
The packaging and container label of immersion oil conforming to this part of ISO 8036 shall be marked with the indication “ISO 8036-1g. Toxic, harmful or suspect components shall be indicated on the packaging and on the container label.
In addition, the following information may be included on the container label:
a) the pcincipal refractive index and its tolerance at 23 °C;
b) the Abbe number and its tolerance at 23 °C;
c) the refractive index temperature coefficient:
d) the viscosity, in metres squared per second, and tolerance;
e) the date of expiry for unopened containers;
f) the manufacturers or supplier’s name or mark, and the country of origin.